About Us
TissueCraft is building a cloud lab focused on improving the efficiency of drug discovery by increasing the predictability, reproducibility, and scalability of cell- and tissue-based assays used in preclinical drug development.
We provide drug developers the power to choose the most reliable drug candidates earlier in the drug discovery process to focus their efforts on developing drugs with high chances of success in clinical trials.
Our commitment to quality is at the core of every product and service we provide from design through delivery and support. We started TissueCraft with the desire to accelerate the identification of new clinically efficacious drugs. We are passionate about our goal and aspire to build infrastructure to facilitate discoveries in life sciences.
Technology
Micropatterned Plate (2D)
Traditional microplates, when used for high-throughput drug screening, have limited to no control over local cell densities and the cellular microenvironment. Cells aggregate around the circumference of the wells, in the center, or a combination of the two. This affects cellular behavior which results in major issues in cellular imaging capabilities, limited reproducibility, and high variability of drug studies. Our micropatterned plates precisely control local cell densities forming uniform cellular microenvironments that enable reproducible, predictive, and scalable drug screening assays. The plates consist of a glass bottom surface and 96-wells that are used for precise positioning of adherent cells. Each well contains an array of micropatterns of various shapes and sizes for protein and cell adhesion enabling highly uniform and reproducible cell seeding to reduce experimental variability.
Microtissue Plate (3D)
Our microtissue plates are capable of quantifying tissue-level functions such as contractile force and are amenable to standard microplate manipulations. The microtissue plate is a 96-well-based array of 3D tissues in custom 3D-print-molded microplates capable of contractile force measurement. Within each well, two elastomeric microcantilevers are situated above a circumferential ramp. The wells are seeded with cell-laden collagen, which, in response to the gradual slope of the circumferential ramp, self-organizes around tip-gated microcantilevers to form contracting tissues. The contractile force exerted by the tissues is measured and calculated using the deflection of the cantilevers. A suite of automated protocols for tissue seeding, image acquisition, and analysis to enable the measurement of contractile force with increased throughput is optional.
Contact Us
We provide industry-leading micropatterning and microtissue technology using a variety of cell types and tissues, including human pluripotent stem cell-derived cell types. For custom assay development, we will work closely with your team to develop technologies and workflows for your specific application. Send us a request and we will partner with you to achieve your goals.
Publications
Micropatterned Plate (2D)
Microtissue Plate (3D)
